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sample ID form.
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sample preparation guidelines.
Pricing
Single Cell Core Pricing – 10X Genomics Chemistries
| Single Cell Library prep -Base (1st sample) 3’ or 5’ | $3,251.00 |
| Single Cell Library prep – (samples 2-8) 3' or 5’ | $2.090.00 |
| Single Cell ATAC-Seq (1st sample) | $3,189.00 |
| Single Cell ATAC-Seq (samples 2-8) | $2,165.00 |
| Single Cell Multiome (1st sample) | $3,626.00 |
| Single Cell Multiome (samples 2-8) | $3,319.00 |
Single Cell Fixed RNA Profiling Assays (FLEX) |
|
| Single Cell FLEX Single-Plex (per sample) | $2,352.00 |
| Single Cell FLEX 4-Plex (4 samples) | $4,182.00 |
| Single Cell FLEX 16-Plex (16 samples) | $10,809.00 |
Library QC Pricing
| TapeStation (per sample) | $32.00 |
| Qubit (each) | $7.00 |
Additional Library Construction Pricing
| TCR/BCR Library | $321.00 |
| Feature Barcoding Library (Cite-Seq/Hash Tagging) | $326.00 |
Miscellaneous Service Pricing
| Single Cell Data analysis (per hour) | $76.00 |
| Single Cell Hourly Service Fee (Late Fee per hour) | $63.00 |
| Antibody Aliquot (per sample) | $52.00 |
| CellPlex Lipid Multiplexing Aliquot | $78.00 |
Investigator has 6 unique samples but is trying to save on costs. They have chosen to use cell hashing and pool their samples into a single library prep.
| Price Per Unit | Units | Cost | |
| First 5Prime Gene Expression Library | $3,251.00 | 1 | $3,251.00 |
| TCR Library | $321.00 | 1 | $321.00 |
| Cell Hashing Library | $326.00 | 1 | $326.00 |
| Cell Hashing Antibody Aliquot | $52.00 | 6 | $312.00 |
| TapeStation | $32.00 | 5 | $160.00 |
| Qubit Quantification | $7.00 | 3 | $21.00 |
| Total Cost of Library Prep (no sequencing, no data analysis): | $4,391.00 | ||
Investigator is running a multiple timepoint study. A single patient sample is collected and submitted to the Core on 3 separate days. Analysis will be provided by the Core.
| Price Per Unit | Units | Cost | |
| First 3PrimeV3 Gene Expression Library | $3251.00 | 3 | $9753.00 |
| CITE Seq Feature Barcoding Library | $326.00 | 3 | $978.00 |
| TapeStation | $32.00 | 9 | $288.00 |
| Qubit Quantification | $7.00 | 6 | $42.00 |
| Data Analysis (Alignment, Cell Ranger Output Basic Clustering) | $76 per hour | 3 hours | $228.00 |
| Total Cost of Library Prep (no sequencing, 3 hours of data analysis): | $11,289.00 | ||
Investigator is comparing control vs experimental mouse samples and is pooling each sample group together to save on costs. Samples were submit after working hours and incurred late fees.
| Price Per Unit | Units | Cost | |
| First 5Prime Gene Expression Library | $3251.00 | 1 | $3251.00 |
| Second 5Prime Gene Expression Library | $2,090.00 | 1 | $2,090.00 |
| Late Fee | $63.00 per hour late | 2 hours late | $126.00 |
| TCR Library | $321.00 | 2 | $642.00 |
| BCR Library | $321.00 | 2 | $642.00 |
| Cell Hashing Library | $326.00 | 2 | $652.00 |
| Cell Hashing Antibody Aliquot | $52.00 | 10 | $520.00 |
| TapeStation | $32.00 | 14 | $448.00 |
| Qubit Quantification | $7.00 | 8 | $56.00 |
| Total Cost of Library Prep (no sequencing, no data analysis): | $8,427.00 | ||
Investigator has 2 samples for ATAC-Seq. Cell hashing cannot be utilized.
| Price Per Unit | Units | Cost | |
| First scATAC-seq Library | $3,189.00 | 1 | $3,189.00 |
| Second scATAC-seq Library | $2,165.00 | 1 | $2,165.00 |
| TapeStation | $32.00 | 2 | $64.00 |
| Qubit Quantification | $7.00 | 2 | $14.00 |
| Data Analysis (Alignment, Cell Ranger Output Basic Clustering) | $76.00 | 5 hours | $380.00 |
| Total Cost of Library Prep (no sequencing, 5 hours of data analysis): | $5,812.00 | ||